Review



emerald-turboid  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Addgene inc emerald-turboid
    Emerald Turboid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emerald-turboid/emerald+turboid/pmc10990277-174-0-31
    Average 90 stars, based on 1 article reviews
    emerald-turboid - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Generated:

    Article Title: Exploitation of the Secretory Pathway by SARS-CoV-2 Envelope
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector. mEmerald was amplified from mEmerald-Sec61b-C1 (Addgene #90992) and TurboID amplified from 3xHA-TurboID-NLS_pCDNA3, with AgeI and EcoRI restriction sites for insertion into pEGFP-C1. pHLARE plasmids were a kind gift from Prof. Diana Barber (University of California, San Francisco).

    Article Title: ER-export and ARFRP1/AP-1–dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector, with mEmerald amplified from mEmerald-Sec61b-C1 (Addgene, no. 90992) and TurboID amplified from 3× HA-TurboID-NLS_pCDNA3, with the assembled construct cut with Age I and Eco RI to replace enhanced GFP (EGFP) in pEGFP-C1 (Addgene, no. 54759) also digested with Age I and Eco RI. pHLARE plasmids were a gift from D. Barber (University of California, San Francisco).

    Plasmid Preparation:

    Article Title: Exploitation of the Secretory Pathway by SARS-CoV-2 Envelope
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector. mEmerald was amplified from mEmerald-Sec61b-C1 (Addgene #90992) and TurboID amplified from 3xHA-TurboID-NLS_pCDNA3, with AgeI and EcoRI restriction sites for insertion into pEGFP-C1. pHLARE plasmids were a kind gift from Prof. Diana Barber (University of California, San Francisco).

    Article Title: ER-export and ARFRP1/AP-1–dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector, with mEmerald amplified from mEmerald-Sec61b-C1 (Addgene, no. 90992) and TurboID amplified from 3× HA-TurboID-NLS_pCDNA3, with the assembled construct cut with Age I and Eco RI to replace enhanced GFP (EGFP) in pEGFP-C1 (Addgene, no. 54759) also digested with Age I and Eco RI. pHLARE plasmids were a gift from D. Barber (University of California, San Francisco).

    Amplification:

    Article Title: Exploitation of the Secretory Pathway by SARS-CoV-2 Envelope
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector. mEmerald was amplified from mEmerald-Sec61b-C1 (Addgene #90992) and TurboID amplified from 3xHA-TurboID-NLS_pCDNA3, with AgeI and EcoRI restriction sites for insertion into pEGFP-C1. pHLARE plasmids were a kind gift from Prof. Diana Barber (University of California, San Francisco).

    Article Title: ER-export and ARFRP1/AP-1–dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector, with mEmerald amplified from mEmerald-Sec61b-C1 (Addgene, no. 90992) and TurboID amplified from 3× HA-TurboID-NLS_pCDNA3, with the assembled construct cut with Age I and Eco RI to replace enhanced GFP (EGFP) in pEGFP-C1 (Addgene, no. 54759) also digested with Age I and Eco RI. pHLARE plasmids were a gift from D. Barber (University of California, San Francisco).

    Construct:

    Article Title: Exploitation of the Secretory Pathway by SARS-CoV-2 Envelope
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector. mEmerald was amplified from mEmerald-Sec61b-C1 (Addgene #90992) and TurboID amplified from 3xHA-TurboID-NLS_pCDNA3, with AgeI and EcoRI restriction sites for insertion into pEGFP-C1. pHLARE plasmids were a kind gift from Prof. Diana Barber (University of California, San Francisco).

    Article Title: ER-export and ARFRP1/AP-1–dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector, with mEmerald amplified from mEmerald-Sec61b-C1 (Addgene, no. 90992) and TurboID amplified from 3× HA-TurboID-NLS_pCDNA3, with the assembled construct cut with Age I and Eco RI to replace enhanced GFP (EGFP) in pEGFP-C1 (Addgene, no. 54759) also digested with Age I and Eco RI. pHLARE plasmids were a gift from D. Barber (University of California, San Francisco).

    Control:

    Article Title: Exploitation of the Secretory Pathway by SARS-CoV-2 Envelope
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector. mEmerald was amplified from mEmerald-Sec61b-C1 (Addgene #90992) and TurboID amplified from 3xHA-TurboID-NLS_pCDNA3, with AgeI and EcoRI restriction sites for insertion into pEGFP-C1. pHLARE plasmids were a kind gift from Prof. Diana Barber (University of California, San Francisco).

    Article Title: ER-export and ARFRP1/AP-1–dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication
    Article Snippet: Emerald-TurboID was used for a cytosolic control in proximity biotinylation experiments and was generated by using HiFi DNA Assembly to assemble Emerald-TurboID in a pLXIN vector, with mEmerald amplified from mEmerald-Sec61b-C1 (Addgene, no. 90992) and TurboID amplified from 3× HA-TurboID-NLS_pCDNA3, with the assembled construct cut with Age I and Eco RI to replace enhanced GFP (EGFP) in pEGFP-C1 (Addgene, no. 54759) also digested with Age I and Eco RI. pHLARE plasmids were a gift from D. Barber (University of California, San Francisco).



    Similar Products

    90
    Addgene inc emerald-turboid
    Emerald Turboid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emerald-turboid/emerald+turboid/pmc10990277-174-0-31
    Average 90 stars, based on 1 article reviews
    emerald-turboid - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results